TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?-标准-资讯-生物在线
·

TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?

作者：Agrisera AB 2020-10-21T00:00 (访问量:1883)

 TBS-T or PBS-T, does it matter which buffer is used for antibody incubations? 

Oftentimes TBS-T is a buffer of choice applied in many Western blot protocols for all antibody incubations. 

However, as you can see in this example, changing to PBS-T has also changed a pattern of detected bands. Analysed samples (Arabidopsis thaliana), protein load/well, primary, secondary antibodies and detection reagent were kept the same. 

Antibody incubation buffer has been changed from TBS-T (left pannel) to PBS-T(right panel).
 


 

   Western blot with anti-catalase antibodies


Western blot protocol with all details for this experiment can be found here. 

 

 

 

Agrisera AB 商家主页

地 址： 21 Vännäs, SWEDEN, Europe

联系人： Dr. Joanna Porankiewicz-Asplund

电 话： 0046704137107

传 真：

Email：61382170@qq.com

相关咨询

Antigen, immunogen and epitope, what is the difference? (2022-05-24T10:12 浏览数:4733)

How to transfer proteins of low molecular weight? (2022-05-24T10:11 浏览数:4884)

How to save time and shorten Western blot protocol? (2022-05-24T10:09 浏览数:3243)

How to get an intense signal in Western blot using a weak antibody? (2022-04-14T17:01 浏览数:7248)

Antigen, immunogen and epitope, what is the difference? (2022-04-14T17:00 浏览数:7023)

What are the benefits of using directly conjugated primary antibodies? (2022-04-14T16:59 浏览数:5518)

How to transfer proteins of low molecular weight? (2022-04-14T16:57 浏览数:4847)

20% discount on loading control and compartment marker antibodies (2022-04-14T16:57 浏览数:4872)

New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors (2022-02-23T10:28 浏览数:902)

New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors (2022-02-23T10:28 浏览数:4170)

ADVERTISEMENT