5-Bromo-2′-deoxyuridine   5-溴-2′-脱氧尿苷

Synonym ：

BUdR | 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil | 5-BrdU | 5-溴-2′-脱氧尿苷

   | 5-Bromouracil deoxyriboside | 5-溴脱氧尿嘧啶核苷 | 5-溴,2’-脱氧尿嘧啶核苷

Descriptions：

   Brdu，中文全名5-溴脱氧尿嘧啶核苷，为胸腺嘧啶的衍生物，可代替胸腺嘧啶在DNA合成期（S期），活体注射或细胞培养加入，而后利用抗Brdu单克隆抗体，ICC染色，显示增殖细胞。同时结合其它细胞标记物，双重染色，可判断增殖细胞的种类，增殖速度，对研究细胞动力学有重要意义。

  目前常用的细胞增殖标记物有4种，Brdu (5-bromo-2--deoxyuridine), DNA polymerase α,PCNA （Proliferating cell nuclear antigen）,Ki-67 等。因组织细胞内无内源性Brdu存在，所以Brdu应用较广。Brdu，中文全名5-溴脱氧尿嘧啶核苷，为胸腺嘧啶的衍生物，可代替胸腺嘧啶在DNA合成期（S期），活体注射或细胞培养加入，而后利用抗Brdu单克隆抗体，ICC染色，显示增殖细胞。同时结合其它细胞标记物，双重染色，可判断增殖细胞的种类，增殖速度，对研究细胞动力学有重要意义。

APPLICATIONS:

BrdU is selectively incorporated into cell DNA at the S phase of cell cycle. The use of BrdU as a thymidine analog has made possible the identification of DNA synthesis in suspensions of cells, cell smears and tissue sections. The incorporation of BrdU into DNA in place of thymidine is discussed by A.L. Givan.5 BrdU at 0.16 to 500 mg/mL of cell culture media produced inhibition of growth of KD cells (rabbit kidney cells). Effective inhibition at concentrations greater than 1.0 mg/mL was observed.6 It is incorporated, in vivo,by injecting 10-100 mg/kg at 10 mg/mL in saline intraperitoneally.7 It is also incorporated into bone marrow cells in culture at a final concentration of 10 mM at 37EC for one hour. For incorporation to occur, the BrdU must be phosphorylated in the cell by thymidine kinase.8 FITC-conjugated second antibodies can be used with antibodies specific for BrdU (Sigma product B2531)

which will make "new" DNA fluoresce green; denatured DNA can be stained with propidium iodide and will fluoresce red.

技术参数：

Product Name   5-Bromo-2′-deoxyuridine,≥99% (HPLC)

Product Number:B5002

Product Brand:Sigma

CAS Number:59-14-3

Molecular Formula:C9H11BrN2O5

Molecular Weight:307.10       

Storage Temp:-20°C

APPEARANCE:WHITE TO OFF-WHITE POWDER

SOLUBILITY:CLEAR COLORLESS TO VERY FAINT YELLOW SOLUTION AT 50MG/ML OF 1MAMMONIUM HYDROXIDE

UV-VIS SPECTRUM:EMM = 8.9 TO 9.2 AT LAMBDA MAX279 TO 280NM AT PH1.0

PURITY BY HPLC:99% MINIMUM

SOLUBILITY / SOLUTION STABILITY:

Sigma routinely tests the solubility at 50 mg/mL in 1 M ammonium hydroxide yielding a clear colorless solution. It can be dissolved in water at a concentration of up to 10 mg/mL. It is also soluble in DMF, DMSO and water (with heat) at 50-100 mg/mL.

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