| Can tubulin be used as a loading control in metal stress? To check this Anti-tubulin alpha chain antibodies were used on extracts from: Nicotiana tabacum plants were treated with silver nanoparticles (AgNPs) with 2 different coatings. Tubulin alpha protein content marked by a blue arrow in the image to the right, was influenced by silver nanoparticle treatment. Similar results were obtained for tubulin gamma chain. Therefore, it can be concluded that: Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as loading control under these conditions. |  All experimental details can be found here. Courtesy of MSc, Karla Košpić, University of Zagreb, Faculty of Science Department of Biology, Croatia |
Tubulin alpha and gamma are not suitable as a loading control for metal stress
作者：Agrisera AB 2021-04-12T00:00 (访问量:2261)
Agrisera AB 商家主页
地 址： 21 Vännäs, SWEDEN, Europe
联系人： Dr. Joanna Porankiewicz-Asplund
电 话： 0046704137107
传 真：
Email：61382170@qq.com
相关咨询
Antigen, immunogen and epitope, what is the difference? (2022-05-24T10:12 浏览数:4711)
How to transfer proteins of low molecular weight? (2022-05-24T10:11 浏览数:4857)
How to save time and shorten Western blot protocol? (2022-05-24T10:09 浏览数:3219)
How to get an intense signal in Western blot using a weak antibody? (2022-04-14T17:01 浏览数:7224)
Antigen, immunogen and epitope, what is the difference? (2022-04-14T17:00 浏览数:6995)
What are the benefits of using directly conjugated primary antibodies? (2022-04-14T16:59 浏览数:5492)
How to transfer proteins of low molecular weight? (2022-04-14T16:57 浏览数:4822)
20% discount on loading control and compartment marker antibodies (2022-04-14T16:57 浏览数:4850)
New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors (2022-02-23T10:28 浏览数:883)
New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors (2022-02-23T10:28 浏览数:4155)
ADVERTISEMENT