Size  100ug
Cat code  200559
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  95kDa
Sensitivity  This antibody detects endogenous levels of EEF2 and does not cross-react with related proteins.
Immunogen  Purified recombinant human EEF2 protein fragments expressed in E.coli.
UniProt Number  P13639
GeneBank ID  NM_001961.3
Subcellular location  Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  eukaryotic translation elongation factor 2;EF2;EEF-2;EF-2;elongation factor 2;polypeptidyl-tRNA translocase
Gene_symbol  EF2; EF-2; EEF-2 
Summary  This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
UniPort summary  Function  Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Application Images :  
Western blot detection of EEF2 in HL-60 cell lysates using EEF2 antibody (1:1000 diluted).
Predicted band size:95KDa
Observed band size: 95KDa.