
pMSCV-IRES-GFP II (pMIG II) 逆病毒载体质粒图谱序列抗性
产品名称: pMSCV-IRES-GFP II (pMIG II) 逆病毒载体质粒图谱序列抗性
英文名称: pMSCV-IRES-GFP II (pMIG II) 逆病毒载体质粒图谱序列抗性说明书价格报价
产品编号: Biovector105802
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
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pMSCV-IRES-GFP II (pMIG II)
(Plasmid #52107)
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PURPOSE(Empty Backbone) a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP
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DEPOSITING LAB
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PUBLICATION
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PRICE$65 (USD)
Add to Cart
Available to Academic and Nonprofits Only
BACKBONE
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Vector backboneMSCV IRES GFP
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Modifications to backboneexpanded MCS
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Vector typeMammalian Expression, Retroviral
- Tag / Fusion Protein
- IRES-EGFP (C terminal on backbone)
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor Sequences
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ sequencing primerpLXSN_5_primer (Common Sequencing Primers)
RESOURCE INFORMATION
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Terms and Licenses
DEPOSITOR COMMENTS
The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.
How to cite this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-IRES-GFP II (pMIG II) was a gift from Dario Vignali (Addgene plasmid # 52107) -
For your References section:
Generation of T-cell receptor retrogenic mice. Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA. Nat Protoc. 2006;1(1):406-17. 10.1038/nprot.2006.61 PubMed 17406263